Year: 2022 | Month: September | Volume 15 | Issue 3
Augmentation of Developmental Competence of Immature Cattle Oocytes Supplemented with Growth Factors in Culture Media
Shiva Prasad
Rohit Kumar
Muthu Karunakaran
Ashok Santra
Subrata K. Das
DOI:10.30954/0974-1712.03.2022.10
Abstract:
The aim of the present study was to produce cattle embryos through in vitro maturation, fertilization and culture by supplementing culture media with insulin-like growth factor-1 individually and in combination with epidermal growth factor. Cattle ovaries were collected from the abattoir and carried to the laboratory within 3-4 h in normal saline, maintaining 30-35 °C. Cumulus oocyte complexes (COCs) were collected from visible surface follicles and matured in-vitro in maturation media supplemented with (i) insulin-like growth factor-1 and (ii) in combination with epidermal growth factor at 38.5 °C in CO2 incubator with maximum humidity. After 24 h matured, oocytes were allowed for fertilization with capacitated sperms in Fert-BO media at 38.5 °C in CO2 incubator. After 15-18 h, oocytes were cultured in 100 µl droplets of mCR2aa medium supplemented with growth factor. After 48 h, cleavage was checked and further co-cultured with oviductal cells for development. In the first experiment, the cleavage rate (63.27±2.17a) and morula formation rate (20.09±2.57a) were significantly (p<0.05) higher in the treatment group @ 50 ng/ml of IGF-1 as compared to the control group. In contrast, in the second experiment the cleavage rate (70.00±2.16a) and morula formation rate (25.30±1.45a) were significantly (p<0.05) higher in the treatment group @ 50 + 10 ng/ml of IGF-1+ EGF, as compared to control group. From the present study, it could be concluded that insulin-like growth factor-1 may have induced cleavage after fertilization and helps in early embryo developmental growth.
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Highlights
- IGF-1 @ 50 ng/ml and in combination with EGF @ 10 ng/ml enhanced the cleavage rate and helped in early embryonic developmental growth significantly.
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